NanoComp report
Summary statistics
| feature |
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| General summary |
WT_C_R1 |
WT_C_R2 |
WT_C_R3 |
WT_AA_R1 |
WT_AA_R2 |
WT_AA_R3 |
anac017_1_C_R1 |
anac017_1_C_R2 |
anac017_1_C_R3 |
anac017_1_AA_R1 |
| Mean read length |
1,012.3 |
959.6 |
1,010.5 |
1,032.1 |
960.9 |
900.4 |
1,046.1 |
997.7 |
957.8 |
1,047.1 |
| Mean read quality |
9.3 |
9.2 |
9.2 |
9.4 |
8.9 |
8.9 |
9.0 |
8.9 |
8.8 |
8.9 |
| Median read length |
887.0 |
844.0 |
887.0 |
947.0 |
833.0 |
791.0 |
919.0 |
876.0 |
860.0 |
920.0 |
| Median read quality |
9.5 |
9.3 |
9.3 |
9.6 |
9.0 |
9.0 |
9.1 |
9.0 |
8.9 |
9.0 |
| Number of reads |
1,258,927.0 |
1,511,111.0 |
1,183,990.0 |
1,281,147.0 |
1,332,072.0 |
1,515,132.0 |
1,651,309.0 |
1,108,328.0 |
357,862.0 |
1,404,090.0 |
| Read length N50 |
1,162.0 |
1,076.0 |
1,155.0 |
1,293.0 |
1,068.0 |
1,004.0 |
1,252.0 |
1,156.0 |
1,083.0 |
1,235.0 |
| STDEV read length |
559.0 |
521.0 |
552.5 |
511.0 |
543.2 |
471.6 |
581.1 |
554.3 |
488.4 |
579.2 |
| Total bases |
1,274,439,587.0 |
1,449,990,920.0 |
1,196,394,780.0 |
1,322,300,002.0 |
1,280,001,710.0 |
1,364,255,477.0 |
1,727,372,885.0 |
1,105,795,587.0 |
342,767,408.0 |
1,470,164,056.0 |
| Number, percentage and megabases of reads above quality cutoffs |
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| >Q10 |
369480 (29.3%) 397.7Mb |
356380 (23.6%) 363.0Mb |
271380 (22.9%) 290.1Mb |
418007 (32.6%) 468.5Mb |
177513 (13.3%) 174.4Mb |
222383 (14.7%) 206.0Mb |
277043 (16.8%) 306.3Mb |
132425 (11.9%) 136.5Mb |
41332 (11.5%) 41.7Mb |
172402 (12.3%) 187.0Mb |
| >Q15 |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
| >Q20 |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
| >Q25 |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
| >Q30 |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
0 (0.0%) 0.0Mb |
| Top 5 highest mean basecall quality scores and their read lengths |
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| 1 |
14.5 (209) |
14.2 (202) |
14.1 (263) |
14.0 (400) |
13.5 (384) |
13.7 (270) |
13.8 (201) |
13.7 (204) |
13.6 (200) |
14.8 (241) |
| 2 |
14.3 (238) |
14.0 (208) |
14.0 (258) |
13.7 (222) |
13.4 (389) |
13.7 (630) |
13.7 (210) |
13.3 (201) |
13.4 (264) |
13.4 (253) |
| 3 |
14.1 (216) |
13.9 (240) |
13.5 (402) |
13.7 (890) |
13.4 (399) |
13.4 (269) |
13.6 (419) |
13.2 (395) |
13.1 (239) |
13.4 (206) |
| 4 |
14.0 (266) |
13.9 (235) |
13.5 (345) |
13.6 (1363) |
13.2 (582) |
13.4 (239) |
13.5 (350) |
13.1 (231) |
13.1 (379) |
13.4 (245) |
| 5 |
13.9 (234) |
13.8 (280) |
13.4 (410) |
13.6 (487) |
13.2 (205) |
13.4 (213) |
13.5 (240) |
13.1 (229) |
12.9 (243) |
13.3 (289) |
| Top 5 longest reads and their mean basecall quality score |
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| 1 |
9939 (10.0) |
14165 (7.9) |
11120 (10.3) |
10367 (10.5) |
11349 (8.9) |
7202 (9.5) |
13335 (9.7) |
10872 (9.4) |
8103 (10.2) |
13921 (9.9) |
| 2 |
9273 (10.0) |
8814 (10.2) |
10467 (10.7) |
9189 (9.1) |
10574 (8.4) |
6908 (7.9) |
12091 (10.1) |
10683 (10.2) |
7347 (8.2) |
13369 (9.2) |
| 3 |
9144 (10.4) |
6993 (8.1) |
9275 (10.0) |
8767 (10.2) |
10036 (9.9) |
6810 (7.5) |
12074 (9.5) |
10575 (8.8) |
7311 (9.9) |
10260 (9.7) |
| 4 |
8918 (10.4) |
6678 (10.3) |
8875 (8.9) |
8737 (8.2) |
9969 (10.7) |
6689 (10.0) |
10437 (10.2) |
9590 (8.2) |
7066 (9.5) |
9801 (9.6) |
| 5 |
8769 (10.1) |
6632 (9.8) |
8445 (9.5) |
8731 (10.1) |
9410 (8.0) |
6582 (8.7) |
10230 (9.7) |
8999 (8.9) |
6845 (9.1) |
9218 (9.8) |
Plots
Comparing number of reads
Comparing throughput in bases
Comparing read length N50
Comparing average base call quality score
Histogram of read lengths
Normalized histogram of read lengths
Weighted histogram of read lengths